Anti-WWOX (phospho Y33)抗体(ab78210)


  • 产品名称Anti-WWOX (phospho Y33)抗体
    参阅全部 WWOX 一抗
  • 描述
    兔多克隆抗体to WWOX (phospho Y33)
  • 特异性This antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human WWOX around the phosphorylation site of tyrosine 33 (W-V-YP-Y-A).

  • 阳性对照
    • Extracts from HepG2 cells. Human colon carcinoma tissue.



Our Abpromise guarantee covers the use of ab78210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.


  • 功能Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • 组织特异性Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • 疾病相关Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
    Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
  • 序列相似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 2 WW domains.
  • 结构域The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
  • 翻译后修饰Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
    Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
  • 细胞定位Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5330426P09Rik antibody
    • 9030416C10Rik antibody
    • Aberrant WW domain-containing oxidoreductase antibody
    • D16S432E antibody
    • EC 1.1.1.- antibody
    • FOR antibody
    • FRA16D antibody
    • Fragile site FRA16D oxidoreductase antibody
    • Fragile site FRA16D Oxireductase antibody
    • HHCMA56 antibody
    • MGC55975 antibody
    • PRO0128 antibody
    • Putative oxidoreductase antibody
    • SDR41C1 antibody
    • Short chain dehydrogenase/reductase family 41C, member 1 antibody
    • WOX1 antibody
    • WW domain containing oxidoreductase antibody
    • WW domain-containing oxidoreductase antibody
    • WW domain-containing protein WWOX antibody
    • wwox antibody
    • WWOX_HUMAN antibody
    • zgc:55975 antibody
    see all

Anti-WWOX (phospho Y33) antibody 图像

  • All lanes : Anti-WWOX (phospho Y33) antibody (ab78210) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins)
    Lane 2 : Extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins) plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ab78210 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

Anti-WWOX (phospho Y33) antibody (ab78210)参考文献

ab78210 has not yet been referenced specifically in any publications.

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