Anti-WWOX (phospho Y33)抗体(ab129881)
Key features and details
- Rabbit polyclonal to WWOX (phospho Y33)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-WWOX (phospho Y33)抗体
参阅全部 WWOX 一抗 -
描述
兔多克隆抗体to WWOX (phospho Y33) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human colon carcinoma tissue; HepG2 cell extracts.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab129881 is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
|
|
IHC-P |
1/50 - 1/100.
|
说明 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. |
IHC-P
1/50 - 1/100. |
靶标
-
功能
Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm. -
组织特异性
Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines. -
疾病相关
Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239]. -
序列相似性
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 2 WW domains. -
结构域
The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1. -
翻译后修饰
Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
Ubiquitinated when phosphorylated by TNK2, leading to its degradation. -
细胞定位
Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 51741 Human
- Entrez Gene: 80707 Mouse
- Omim: 605131 Human
- SwissProt: Q9NZC7 Human
- SwissProt: Q91WL8 Mouse
- Unigene: 461453 Human
- Unigene: 440420 Mouse
-
别名
- 5330426P09Rik antibody
- 9030416C10Rik antibody
- Aberrant WW domain-containing oxidoreductase antibody
see all
图片
-
ab129881, at 1/50 dilution, staining WWOX in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry, in the absence (left) and presence (right) of the phosphopeptide.
-
All lanes : Anti-WWOX (phospho Y33) antibody (ab129881) at 1/500 dilution
Lane 1 : HepG2 cell extract, treated with PMA
Lane 2 : HepG2 cell extract, treated with PMA with synthesized Phosphopeptide
Predicted band size: 47 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab129881 尚未被引用在任何文献中。