The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 18597617
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 171 kDa).
Use at an assay dependent concentration.
Use a concentration of 10 - 20 µg/ml.
功能Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
组织特异性Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
疾病相关Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Bromodomain adjacent to zinc finger domain protein 1B antibody
hWALP 2 antibody
transcription factor WSTF antibody
Tyrosine-protein kinase BAZ1B antibody
WBRS 9 antibody
WBSC 10 antibody
Williams Beuren syndrome chromosome region 9 protein antibody
Williams syndrome transcription factor antibody
Williams-Beuren syndrome chromosomal region 10 protein antibody
Williams-Beuren syndrome chromosomal region 9 protein antibody
Anti-WSTF antibody - Carboxyterminal end 图像
Western blot - WSTF antibody - Carboxyterminal end (ab50632)
Lane 1 : Anti-WSTF antibody - Carboxyterminal end (ab50632) at 2 µg/ml Lanes 2, 4 & 5 : Anti-WSTF antibody - Carboxyterminal end (ab50632) at 1 µg/ml Lane 3 : Anti-WSTF antibody - Carboxyterminal end (ab50632) at 0.5 µg/ml
Lane 1 : Mcf7 cell lysate Lane 2 : Mcf7 cell lysate Lane 3 : Mcf7 cell lysate Lane 4 : Mcf7 cell lysate with non-relevant peptide
at 10 µg/ml Lane 5 : Mcf7 cell lysate with WSTF immunizing peptide
at 10 µg/ml