Anti-WSTF抗体(ab87325)

概述

  • 产品名称Anti-WSTF抗体
    参阅全部 WSTF 一抗
  • 描述
    兔多克隆抗体to WSTF
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of Human WSTF (SwissProt Q9UIG0).

  • 阳性对照
    • Human Breast Carcinoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma and Placenta tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab87325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
    • 组织特异性Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
    • 疾病相关Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 序列相似性Belongs to the WAL family. BAZ1B subfamily.
      Contains 1 bromo domain.
      Contains 1 DDT domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 WAC domain.
    • 发展阶段Expressed at equal levels in 19-23 weeks old fetal tissues.
    • 结构域The N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
      The bromo domain mediates the specific interaction with acetylated histones.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.
    • Information by UniProt
    • 数据库链接
    • 别名
      • baz1b antibody
      • BAZ1B_HUMAN antibody
      • Bromodomain adjacent to zinc finger domain protein 1B antibody
      • hWALP 2 antibody
      • hWALP-2 antibody
      • hWALP2 antibody
      • transcription factor WSTF antibody
      • Tyrosine-protein kinase BAZ1B antibody
      • WALP-2 antibody
      • WALP2 antibody
      • WBRS 9 antibody
      • WBRS-9 antibody
      • WBRS9 antibody
      • WBSC 10 antibody
      • WBSC-10 antibody
      • WBSC10 antibody
      • WBSCR10 antibody
      • WBSCR9 antibody
      • Williams Beuren syndrome chromosome region 9 protein antibody
      • Williams syndrome transcription factor antibody
      • Williams-Beuren syndrome chromosomal region 10 protein antibody
      • Williams-Beuren syndrome chromosomal region 9 protein antibody
      • WSTF antibody
      see all

    Anti-WSTF antibody 图像

    • ab87325, at 1/250 dilution, staining WSTF in formalin-fixed, paraffin-embedded Human non-small cell lung cancer by Immunohistochemistry using DAB staining.

    Anti-WSTF antibody (ab87325)参考文献

    ab87325 has not yet been referenced specifically in any publications.

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    In general, our antibodies are recommended for storage at 4C when they are known to be particularly susceptible to freeze / thaw cycles. All antibodies will lose a small amount of specificity or signal intensity e...

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    Application Immunohistochemistry (Frozen sections)
    Sample Chicken Tissue sections (sectioned embryo)
    Specification sectioned embryo
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton X in PBS
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    提交于 Jan 12 2012

    DISCOUNT CODE: *** Expiration date: February 18th, 2012 I am very pleased to hear you would like to accept our offer and test ab87325 in chicken. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, pl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"