概述

  • 产品名称
    Anti-WSCD1抗体
  • 描述
    兔多克隆抗体to WSCD1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence corresponding to amino acids 257-363 of Human WSCD1.

  • 阳性对照
    • Human colon tissue; Transfected HEK293T cell WSCD1 over-expression lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121491 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121491, at 1/75 dilution, staining WSCD1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-WSCD1 antibody (ab121491) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : WSCD1-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique

文献

ab121491 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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