概述

  • 产品名称
  • 描述
    兔多克隆抗体to WRCH1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    A synthetic peptide corresponding to a region of Human WRCH1.

  • 阳性对照
    • Fetal muscle lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound.
  • 组织特异性
    Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rho family.
  • 细胞定位
    Cell membrane. Golgi apparatus membrane. Cell junction > focal adhesion. Cell projection > podosome. Localizes to podosomes in SRC-transformed cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHU antibody
    • CDC42 L1 antibody
    • CDC42 like GTPase antibody
    • CDC42-like GTPase 1 antibody
    • CDC42L1 antibody
    • G28K antibody
    • GTP binding protein like 1 antibody
    • GTP binding protein SB128 antibody
    • GTP-binding protein-like 1 antibody
    • hG28K antibody
    • mG28K antibody
    • Ras homolog gene family member U antibody
    • Ras homolog gene family member U isoform CRA a antibody
    • Ras like gene family member U antibody
    • Rho GTPase like protein ARHU antibody
    • Rho GTPase-like protein ARHU antibody
    • Rho related GTP binding protein RhoU antibody
    • Rho-related GTP-binding protein RhoU antibody
    • Rhou antibody
    • RHOU_HUMAN antibody
    • Ryu GTPase antibody
    • Wnt-1 responsive Cdc42 homolog 1 antibody
    • Wnt1 responsive Cdc42 homolog antibody
    • WRCH 1 antibody
    • WRCH-1 antibody
    see all

图片

  • Anti-WRCH1 antibody (ab80315) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Bhavsar PJ  et al. Analysis of Rho GTPase expression in T-ALL identifies RhoU as a target for Notch involved in T-ALL cell migration. Oncogene : (2012). Read more (PubMed: 22349824) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A431 cell line)
Loading amount
20 µg
Specification
A431 cell line
Gel Running Conditions
Reduced Denaturing (exponential gradient gel 5-20%)
Blocking step
TBS milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

提交于 Mar 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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