概述

  • 产品名称
  • 描述
    兔多克隆抗体to Wnt9b
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Wnt9b.

    (Peptide available as ab74751.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Liver (Mouse), Kidney (Mouse) And the following whole cell lysate: HepG2

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).

靶标

  • 相关性
    Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
  • 细胞定位
    Secreted: extracellular space: extracellular matrix.
  • 数据库链接
  • 别名
    • MGC124412 antibody
    • Protein Wnt 9b precursor antibody
    • Protein Wnt-14b antibody
    • Protein Wnt-9b antibody
    • Wingless type MMTV integration site 9B antibody
    • Wingless type MMTV integration site family member 15 antibody
    • Wingless type MMTV integration site family member 9B antibody
    • Wnt 14b antibody
    • Wnt-15 antibody
    • Wnt14b antibody
    • Wnt15 antibody
    • Wnt9b wingless type MMTV integration site family member 9B antibody
    see all

Anti-Wnt9b antibody 图像

  • All lanes : Anti-Wnt9b antibody (ab69287) at 1 µg/ml

    Lane 1 : HepG2 Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

实验方案

Anti-Wnt9b antibody (ab69287)参考文献

ab69287 has not yet been referenced specifically in any publications.

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