Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Wnt8a.
This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Heart; E14 Embryonic Brain; E16 Embryonic Brain; E18 Embryonic Brain; E12 Embryonic Brain and Spinal Cord.
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Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Belongs to the Wnt family.
Secreted > extracellular space > extracellular matrix.
Information by UniProt
Protein Wnt-8a antibody
Protein Wnt-8d antibody
wingless type MMTV integration site family, member 8A antibody
Western blot - Anti-Wnt8a antibody (ab110672)
All lanes :
Anti-Wnt8a antibody (ab110672) at 1 µg/ml
Lane 1 :
Brain (Mouse) Tissue Lysate
Lane 2 :
Heart (Mouse) Tissue Lysate
Lane 3 :
E14 Mouse Embryo Brain Tissue Lysate
Lane 4 :
E16 Ms Embryo Brain Tissue Lysate
Lane 5 :
E18 Ms Embryo Brain Tissue Lysate
Lane 6 :
E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
42 kDa (
why is the actual band size different from the predicted?
Additional bands at:
14 kDa, 87 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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