概述
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产品名称Anti-Wnt8a抗体
参阅全部 Wnt8a 一抗 -
描述兔多克隆抗体to Wnt8a
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Mouse
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Wnt8a.
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阳性对照
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Heart; E14 Embryonic Brain; E16 Embryonic Brain; E18 Embryonic Brain; E12 Embryonic Brain and Spinal Cord.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab110672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
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序列相似性Belongs to the Wnt family.
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细胞定位Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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数据库链接
- Entrez Gene: 20890 Mouse
- SwissProt: Q64527 Mouse
- Unigene: 558 Mouse
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别名
- Protein Wnt-8a antibody
- Protein Wnt-8d antibody
- wingless type MMTV integration site family, member 8A antibody
see all
图片
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Western blot - Anti-Wnt8a antibody (ab110672)All lanes : Anti-Wnt8a antibody (ab110672) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
Lane 4 : E16 Ms Embryo Brain Tissue Lysate
Lane 5 : E18 Ms Embryo Brain Tissue Lysate
Lane 6 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 42 kDa (why is the actual band size different from the predicted?)
Additional bands at: 14 kDa, 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
文献
ab110672 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"