概述

  • 产品名称
  • 描述
    兔多克隆抗体to Wnt6
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Wnt6.

    (Peptide available as ab50029.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line); Jurkat (Human T cell lymphoblast-like cell line); A431 (Human epithelial carcinoma cell line); HEK293 (Human embryonic kidney cell line); HepG2 (Human hepatocellular liver carcinoma cell line); MCF7 (Human breast adenocarcinoma cell line); SHSY-5Y (Human neuroblastoma cell line); U2OS (Human osteosarcoma cell line)

性能

应用

Our Abpromise guarantee covers the use of ab50030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 序列相似性
    Belongs to the Wnt family.
  • 翻译后修饰
    Palmitoylation at Ser-228 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-76. Palmitoylation is necessary for proper trafficking to cell surface.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein Wnt 6 antibody
    • Protein Wnt-6 antibody
    • Protein Wnt-6 precursor antibody
    • Wingless type MMTV integration site family, member 6 antibody
    • wnt 6 antibody
    • Wnt6 antibody
    • WNT6_HUMAN antibody
    see all

图片

  • All lanes : Anti-Wnt6 antibody (ab50030) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab50030 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50030, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Zhang L  et al. Increased WNT6 expression in tumor cells predicts unfavorable survival in esophageal squamous cell carcinoma patients. Int J Clin Exp Pathol 8:11421-7 (2015). Read more (PubMed: 26617869) »
  • Richards MH  et al. Porcupine is not required for the production of the majority of Wnts from primary human astrocytes and CD8+ T cells. PLoS One 9:e92159 (2014). WB . Read more (PubMed: 24647048) »

See all 4 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Endometrium, skin and brain (normal and tumor) sam)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM, pH=6)
Permeabilization
No
Specification
Endometrium, skin and brain (normal and tumor) sam
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Formaldehyde
Username

Dr. Céline Gonçalves

Verified customer

提交于 Sep 18 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Dec 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293, SW620, MCF7, HeLa)
Loading amount
20 µg
Specification
293, SW620, MCF7, HeLa
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
5% milk in PBS + 0.1% Tween as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jul 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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