概述
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产品名称Anti-Wnt5b抗体[EPR7135(B)]
参阅全部 Wnt5b 一抗 -
描述兔单克隆抗体[EPR7135(B)] to Wnt5b
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宿主Rabbit
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经测试应用适用于: WB, Flow Cytmore details
不适用于: ICC,IHC-P or IP -
种属反应性与反应: Human
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免疫原
Synthetic peptide corresponding to residues near the C-terminus of Human Wnt5b.
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阳性对照
- HT-1376 cell lysate, HepG2 cell lysate, 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.
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常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR7135(B)
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同种型IgG
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研究领域
相关产品
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab124818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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靶标
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功能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
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序列相似性Belongs to the Wnt family.
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细胞定位Secreted > extracellular space > extracellular matrix.
- Information by UniProt
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数据库链接
- Entrez Gene: 81029 Human
- Omim: 606361 Human
- SwissProt: Q9H1J7 Human
- Unigene: 306051 Human
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别名
- MGC2648 antibody
- Protein Wnt-5b antibody
- Wingless type MMTV integration site family, member 5B antibody
see all
图片
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![Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)](http://www.abcam.cn/ps/datasheet/images/124/ab124818/WNT5B-Primary-antibodies-ab124818-1.jpg)
Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa
文献
ab124818 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"