The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
1/1000. Predicted molecular weight: 39 kDa.
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330].
Belongs to the Wnt family.
Secreted > extracellular space > extracellular matrix.
Wingless type MMTV integration site family member 4 precursor antibody
WNT 4 antibody
WNT 4 protein precursor antibody
Immunohistochemistry (Frozen sections) - Anti-Wnt4 antibody (ab91226)Image is courtesy of an anonymous AbReview.
Immunohistochemical analysis of paraformaldehyde-fixed frozen murine thymus tissue sections, labelling Wnt4 with ab91226 at a dilution of 1/20 incubated for 72 hours at 25°C in a 1% BSA & 3% donkey serum solution in PBST. Permeabilsation was with PBST including 0.1% Triton X-100. Blocking was with the BSA & donkey serum ab91226 was diluted in, incubated for 1 hour at 25°C. The secondary used was a donkey anti-rabbit polyclonal Alexa Fluor® 488
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Wnt4 with ab91226. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.
ICC/IF image of ab91226 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Anti-Wnt4 antibody (ab91226)
Flow cytometry analysis of MDA-MB468 cells labelling Wnt4 (green) with ab91226 compared to a negative control (blue). A FITC-conjugated goat anit-rabbit IgG was used as the secondary antibody.