概述

  • 产品名称Anti-Wnt3a抗体(Biotin)
    参阅全部 Wnt3a 一抗
  • 描述
    兔多克隆抗体to Wnt3a (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human),>98% pure

  • 常规说明The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

性能

应用

Our Abpromise guarantee covers the use of ab54479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at a concentration of 0.25 - 1.0 µg/ml. Use 100µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 39 kDa. The detection limit is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
    • 组织特异性Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
    • 序列相似性Belongs to the Wnt family.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Protein Wnt 3a Precursor antibody
      • Protein Wnt-3a antibody
      • Wingless type MMTV integration site family member 3A antibody
      • Wnt 3a antibody
      • wnt3a antibody
      • WNT3A protein antibody
      • WNT3A_HUMAN antibody
      see all

    Anti-Wnt3a antibody (Biotin) (ab54479)参考文献

    This product has been referenced in:
    • Ling L  et al. Synergism between Wnt3a and heparin enhances osteogenesis via a phosphoinositide 3-kinase/Akt/RUNX2 pathway. J Biol Chem 285:26233-44 (2010). Read more (PubMed: 20547765) »
    • Kwon HS  et al. Myocilin is a modulator of Wnt signaling. Mol Cell Biol 29:2139-54 (2009). ELISA ; Mouse . Read more (PubMed: 19188438) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"