概述

  • 产品名称
  • 描述
    兔多克隆抗体to Wnt3a
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Xenopus laevis
    预测可用于: Chicken, Human, Zebrafish, a wide range of other species
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Wnt3a.

    (Peptide available as ab23326)

  • 阳性对照
    • In WB, this antibody gave a positive signal when tested against a partial recombinant protein. IHC-P: Mouse placenta FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab19925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Can be blocked with Wnt3a peptide (ab23326).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • IHC image of Wnt3a staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19925, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • Anti-Wnt3a antibody (ab19925) at 1 µg/ml + Recombinant mouse Wnt3a, AAs 19-352 with a 16 AA n-terminal non-Wnt3a sequence at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/15000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    ab19925 detects a band of the expected size for recombinant mouse Wnt3a.

文献

This product has been referenced in:
  • Lin S  et al. The chromatin-remodeling enzyme BRG1 promotes colon cancer progression via positive regulation of WNT3A. Oncotarget 7:86051-86063 (2016). WB, IHC ; Human . Read more (PubMed: 27852072) »
  • Li C & Siragy HM High glucose induces podocyte injury via enhanced (pro)renin receptor-Wnt-ß-catenin-snail signaling pathway. PLoS One 9:e89233 (2014). WB ; Mouse . Read more (PubMed: 24533170) »

See all 6 Publications for this product

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (airway tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization
No
Specification
airway tissue
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 27 2016

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Zebrafish Tissue sections (Whole mount (whole zebrafish larvae) and sections)
Specification
Whole mount (whole zebrafish larvae) and sections
Username

Dr. Chrissy Hammond

Verified customer

提交于 Nov 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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