概述

  • 产品名称Anti-Wnt3a抗体[3A6]
    参阅全部 Wnt3a 一抗
  • 描述
    小鼠单克隆抗体[3A6] to Wnt3a
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).

  • 阳性对照
    • A549 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab81614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • 组织特异性Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • 序列相似性Belongs to the Wnt family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein Wnt 3a Precursor antibody
    • Protein Wnt-3a antibody
    • Wingless type MMTV integration site family member 3A antibody
    • Wnt 3a antibody
    • wnt3a antibody
    • WNT3A protein antibody
    • WNT3A_HUMAN antibody
    see all

Anti-Wnt3a antibody [3A6] 图像

  • Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution + A549 lysate at 20 µg

    Secondary
    Goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

  • ab81614 staining Wnt3a in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining procedure was used for detection.

Anti-Wnt3a antibody [3A6] (ab81614)参考文献

This product has been referenced in:
  • Chen F  et al. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. J Surg Res 192:298-304 (2014). Read more (PubMed: 25033705) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 17 2014

Here are the answers to your questions.


1. Are there high resolution images available for the 2 IHC stainings shown on the website?
-I’ve attached higher resolution pictures as requested.

2. Have any additional sec...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"