Anti-Wnt3抗体(ab50341)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Wnt3
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Dog
  • 免疫原

    Synthetic peptide:

    CGRGHNTRTEKRKEK

    , corresponding to internal sequence amino acids 315-329 of Human Wnt3

  • 阳性对照
    • WB: Human brain lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).
ELISA 1/2000.
IHC-P Use a concentration of 2 - 4 µg/ml.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • 疾病相关
    Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.
  • 序列相似性
    Belongs to the Wnt family.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Int 4 antibody
    • INT4 antibody
    • MGC131950 antibody
    • MGC138321 antibody
    • MGC138323 antibody
    • Proto-oncogene Int-4 homolog antibody
    • Proto-oncogene Wnt-3 antibody
    • wingless type MMTV integration site family member 3 antibody
    • Wnt 3 proto oncogene protein antibody
    • WNT 3 proto oncogene protein precursor antibody
    • wnt3 antibody
    • WNT3_HUMAN antibody
    see all

Anti-Wnt3 antibody 图像

  • ab50341 (2µg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.

  • Anti-Wnt3 antibody (ab50341) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).

    Predicted band size : 40 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab50341 at 3.8µg/ml staining Wnt3 in Human breast tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. An AP staining procedure was used.

Anti-Wnt3 antibody (ab50341)参考文献

ab50341 has not yet been referenced specifically in any publications.

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