RabMAb

Anti-Wnt2抗体[EPR3101(2)] (ab109222)

概述

  • 产品名称Anti-Wnt2抗体[EPR3101(2)]
    参阅全部 Wnt2 一抗
  • 描述
    兔单克隆抗体[EPR3101(2)] to Wnt2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Wnt2

  • 阳性对照
    • Jurkat,MCF-7,T47-D, and SKBR-3 cell lysates Human fetal lung tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa).

靶标

  • 功能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 组织特异性Expressed in brain in the thalamus, in fetal and adult lung and in placenta.
  • 序列相似性Belongs to the Wnt family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 形式The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis.
  • 别名
    • Int 1 related protein antibody
    • Int-1-like protein 1 antibody
    • Int-1-related protein antibody
    • INT1L 1 antibody
    • INT1L1 antibody
    • IRP antibody
    • IRP protein antibody
    • ONCOGENE INT1 LIKE 1 antibody
    • Protein Wnt-2 antibody
    • Secreted growth factor antibody
    • Wingless type MMTV integration site family member 2 antibody
    • WNT 2 antibody
    • WNT2 antibody
    • WNT2_HUMAN antibody
    see all

Anti-Wnt2 antibody [EPR3101(2)] 图像

  • Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 40 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (purified) + Rat brain tissue lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 40 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • All lanes : Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : SKBR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 40 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • All lanes : Anti-Wnt2 antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat cell lysates
    Lane 2 : MCF-7 cell lysates
    Lane 3 : T47-D cell lysates
    Lane 4 : SKBR-3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 40 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-Wnt2 antibody [EPR3101(2)] (ab109222)参考文献

This product has been referenced in:
  • Zhang Z  et al. Association of Wnt2 and sFRP4 expression in the third trimester placenta in women with severe preeclampsia. Reprod Sci 20:981-9 (2013). Read more (PubMed: 23322712) »

See 1 Publication for this product

Product Wall

Thank you very much for providing the images and protocol info.

I have checked different publications and databases; all show cytoplasmic staining. The links to the few are attached below;

http://www.proteinatlas.org/ENSG00000105989...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Thank you for contacting Abcam. The peptide maps between amino acids ********* of human Wnt2 (UniProt:  P09544). We have not yet tested whether this antibody will react with other WNT family members, but I did Blast the immunogen sequence and it did no...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"