Anti-Wnt16抗体(ab64461)

概述

  • 产品名称Anti-Wnt16抗体
    参阅全部 Wnt16 一抗
  • 描述
    兔多克隆抗体to Wnt16
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: KRKMRRREKD QRKIPIHKDD LLYVNKSPNY CVEDKKLGIP GTQGRECNRT, corresponding to C terminal amino acids 264-313 of Human Wnt16

  • 阳性对照
    • HepG2 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1:312500.

靶标

  • 功能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 组织特异性Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
  • 序列相似性Belongs to the Wnt family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein Wnt 16 precursor antibody
    • Protein Wnt-16 antibody
    • Wingless type MMTV integration site family member 16 antibody
    • WNT16 antibody
    • WNT16_HUMAN antibody
    see all

Anti-Wnt16 antibody 图像

  • Anti-Wnt16 antibody (ab64461) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-Wnt16 antibody (ab64461)参考文献

ab64461 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab64461 Anti-Wnt16 antibody has never been tested in functional blocking experiments. All tested and guaranteed applications are specified on our datasheets, and the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"