Anti-Wnt10b抗体(ab70816)
Key features and details
- Rabbit polyclonal to Wnt10b
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Wnt10b抗体
参阅全部 Wnt10b 一抗 -
描述
兔多克隆抗体to Wnt10b -
宿主
Rabbit -
特异性
ab70816 only recognizes the longer isoform of Wnt10b and will not cross-react with Wnt10a. -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
15 amino acid peptide from near the center of human Wnt10b (NP_003385).
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阳性对照
- Human skeletal muscle tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70816于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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WB | (2) |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). |
靶标
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功能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
组织特异性
Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain. -
疾病相关
Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. -
序列相似性
Belongs to the Wnt family. -
发展阶段
Infant brain has higher levels of WNT10B than adult brain. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 7480 Human
- Omim: 601906 Human
- SwissProt: O00744 Human
- Unigene: 91985 Human
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别名
- Protein Wnt-10b antibody
- Protein Wnt-12 antibody
- SHFM6 antibody
see all
图片
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Lane 1 : Anti-Wnt10b antibody (ab70816) at 2 µg/ml
Lane 2 : Anti-Wnt10b antibody (ab70816) at 4 µg/ml
All lanes : Human skeletal muscle tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 43 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 93 kDa. We are unsure as to the identity of these extra bands. -
ab70816 at 2.5 µ/ml staining Wnt10 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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ICC/IF image of ab70816 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70816, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab70816 被引用在 22 文献中.
- Li C et al. lncRNA HOTAIRM1 regulates cell proliferation and the metastasis of thyroid cancer by targeting Wnt10b. Oncol Rep 45:1083-1093 (2021). PubMed: 33650656
- Zhai X et al. Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice. Clin Cosmet Investig Dermatol 14:527-539 (2021). PubMed: 34040410
- Cai Y et al. Gigantol inhibits cell proliferation and induces apoptosis by regulating DEK in non-small cell lung cancer. Exp Ther Med 22:1317 (2021). PubMed: 34630671
- Yang Y et al. CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p. Cancer Cell Int 21:651 (2021). PubMed: 34872582
- Zhuang J et al. Extracellular vesicles engineered with valency-controlled DNA nanostructures deliver CRISPR/Cas9 system for gene therapy. Nucleic Acids Res 48:8870-8882 (2020). PubMed: 32810272