Anti-Wnt10a抗体(ab106522)

概述

  • 产品名称Anti-Wnt10a抗体
    参阅全部 Wnt10a 一抗
  • 描述
    兔多克隆抗体to Wnt10a
  • 特异性Despite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 14 amino acid synthetic peptide from near the C terminus of Human Wnt10a (UniProt Q9GZT5).

  • 阳性对照
    • RAW264.7 cell lysate; Human skeletal muscle tissue

性能

应用

Our Abpromise guarantee covers the use of ab106522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • 疾病相关Defects in WNT10A are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. Note=Most patients carrying WNT10A mutations present with sweating anomalies. However, comparison with EDA cases harboring mutations in the ectodysplasin pathway identifies some phenotypic differences. Dermatological features (anomalies of hair and sweat glands) are less severe in patients carrying WNT10A mutations and facial dysmorphism can be absent. The dental phenotype consists in microdontia, whereas teeth agenesis is more frequent in patients carrying mutations in the ectodysplasin pathway.
    Defects in WNT10A are a cause of odonto-onycho-dermal dysplasia (OODD) [MIM:257980]. OODD is a rare autosomal recessive ectodermal dysplasia in which the presenting phenotype is dry hair, severe hypodontia, smooth tongue with marked reduction of fungiform and filiform papillae, onychodysplasia, keratoderma and hyperhidrosis of palms and soles, and hyperkeratosis of the skin.
    Defects in WNT10A are a cause of Schopf-Schulz-Passarge syndrome (SSPS) [MIM:224750]. SSPS is rare ectodermal dysplasia, characterized chiefly by cysts of the eyelid margins, palmoplantar keratoderma, hypodontia, hypotrichosis and nail dystrophy. Multiple eyelid apocrine hidrocystomas are the hallmark of this condition, although they usually appear in adulthood. The concomitant presence of eccrine syringofibroadenoma in most patients and of other adnexal skin tumors in some affected subjects indicates that Schopf-Schulz-Passarge syndrome is a genodermatosis with skin appendage neoplasms.
  • 序列相似性Belongs to the Wnt family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ14301 antibody
    • OODD antibody
    • Protein Wnt-10a antibody
    • SSPS antibody
    • STHAG4 antibody
    • Wingless type MMTV integration site family member 10A antibody
    • WN10A_HUMAN antibody
    • wnt10a antibody
    see all

Anti-Wnt10a antibody 图像

  • Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/ml
    Lane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/ml

    Lane 1 : RAW264.7 cell lysate
    Lane 2 : RAW264.7 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 46 kDa
  • ab106522, at 10 µg/ml, staining Wnt10a in Human skeletal muscle tissue by Immunohistochemistry.
  • Immunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.

    Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.

Anti-Wnt10a antibody (ab106522)参考文献

ab106522 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bladder)
Specification Bladder
Fixative 10% Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Citrate Buffer, PH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C
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提交于 Oct 22 2012

Thank you for taking time to inform us of the problems you have encountered with the anti-Wnt10a antibody (ab106522). I am sorry to hear this antibody is not providing satisfactory results.

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