概述

  • 产品名称
  • 描述
    兔多克隆抗体to Wnt1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • 阳性对照
    • Breast carcinoma. NIH/3T3 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab15251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

靶标

  • 功能
    Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • 序列相似性
    Belongs to the Wnt family.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BMND16 antibody
    • INT1 antibody
    • OI15 antibody
    • oncogene Int1 antibody
    • Proto oncogene protein Wnt 1 antibody
    • Proto-oncogene Int-1 homolog antibody
    • Proto-oncogene Wnt-1 antibody
    • Wingless type MMTV integration site family member 1 antibody
    • wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody
    • Wnt 1 antibody
    • wnt1 antibody
    • WNT1_HUMAN antibody
    see all

图片

  • Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

  • ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);

文献

This product has been referenced in:
  • Kong LY  et al. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/ß-catenin signaling pathway. Oncotarget 8:15507-15519 (2017). WB ; Human . Read more (PubMed: 28099945) »
  • Chen Y  et al. miRNA-148a serves as a prognostic factor and suppresses migration and invasion through Wnt1 in non-small cell lung cancer. PLoS One 12:e0171751 (2017). Read more (PubMed: 28199399) »

See all 31 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (CDK8/CycC)
Specification
CDK8/CycC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Type
Direct
Username

Abcam user community

Verified customer

提交于 Mar 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
35 µg
Sample
Mouse Cell lysate - whole cell (primary mouse pancreatic cancer cell lines)
Specification
primary mouse pancreatic cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (PanIN lesions Pancreas)
Specification
PanIN lesions Pancreas
Fixative
Acetone
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (CaPan1 and Panc1)
Loading amount
35 µg
Specification
CaPan1 and Panc1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreatic tumor)
Specification
Pancreatic tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Podocytes)
Loading amount
30 µg
Specification
Podocytes
Treatment
5ng/ml TGF-beta 5min to 4hr
Gel Running Conditions
Reduced Denaturing (85 gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

提交于 Feb 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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