这个 快线抗体 还未被充份测试和鉴定,因此它受制于相关的 使用条款

概述

性能

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa). Lysates from transfected HEK293 cells expressing Myc-WNK2 as well as endogenous WNK2
ELISA Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab30698).

靶标

  • 相关性
    WNK2 a putative tumour suppressor is a member of a family of serine / threonine protein kinases implicated in the hereditary form of hypertension, pseudohypoaldosteronism type II.
  • 细胞定位
    Cell Membrane and Cytoplasmic
  • 数据库链接
  • 别名
    • Antigen NY-CO-43 antibody
    • KIAA1760 antibody
    • mitogen-activated protein kinase kinase kinase antibody
    • NY CO 43 antibody
    • P/OKcl.13 antibody
    • PRKWNK2 antibody
    • Protein kinase lysine deficient 2 antibody
    • Protein kinase with no lysine 2 antibody
    • SDCCAG43 antibody
    • Serine/Threonine protein kinase WNK2 antibody
    • Serologically defined colon cancer antigen 43 antibody
    • WNK lysine deficient protein kinase 2 antibody
    see all

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Top blot: anti Myc-Tag antibody; Bottom Blot: ab28852 (1/500)

    Lane 1 : Hek293 cell lysate Transfected with empty vector
    Lane 2 : Hek293 cell lysate Transfected with MYC-WNK1
    Lane 3 : Hek293 cell lysate Transfected with MYC-WNK2
    Lane 4 : Hek293 cell lysate Transfected with MYC-WNK3
    Lane 5 : Hek293 cell lysate Transfected with MYC-WKN4

    Secondary
    Goat anti-rabbit IgG conjugated to HRP at 1/3000 dilution
    Developed using the ECL technique

    Predicted band size : 300 kDa

    This image is courtesy of an abreview submitted by Peter Jordan

    Anti-tubulin antibody used as loading control for both blots.

    See Abreview

实验方案

文献

This product has been referenced in:
  • Roy A  et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol 308:F366-76 (2015). Read more (PubMed: 25477473) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (human embryonic kidney (HEK293) cells)
Loading amount
400000 cells
Specification
human embryonic kidney (HEK293) cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Peter Jordan

Verified customer

提交于 Mar 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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