概述

  • 产品名称Anti-WNK1 (phospho T58)抗体
    参阅全部 WNK1 一抗
  • 描述
    兔多克隆抗体to WNK1 (phospho T58)
  • 特异性ab53137 detects endogenous levels of WNK1 only when phosphorylated at threonine 58.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).

  • 阳性对照
    • 293 cell extracts treated with EGF (200ng/ml, 30mins) and human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab53137 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • 组织特异性Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • 疾病相关Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Erythrocyte 65 kDa protein antibody
    • HSAN2 antibody
    • HSN2 antibody
    • hWNK1 antibody
    • KDP antibody
    • KIAA0344 antibody
    • Kinase deficient protein antibody
    • MGC163339 antibody
    • MGC163341 antibody
    • p65 antibody
    • PRKWNK1 antibody
    • Prostate derived sterile 20 like kinase antibody
    • Protein kinase lysine deficient 1 antibody
    • Protein kinase lysine-deficient 1 antibody
    • Protein kinase with no lysine 1 antibody
    • PSK antibody
    • Serine/threonine protein kinase WNK1 antibody
    • Serine/threonine-protein kinase WNK1 antibody
    • With no K antibody
    • WNK lysine deficient protein kinase 1 antibody
    • WNK1 antibody
    • WNK1_HUMAN antibody
    see all

Anti-WNK1 (phospho T58) antibody 图像

  • All lanes : Anti-WNK1 (phospho T58) antibody (ab53137) at 1/300 dilution

    Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30mins)
    Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide


    Predicted band size : 251 kDa
  • ab53137 at 1/50 dilution staining WNK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-WNK1 (phospho T58) antibody (ab53137)参考文献

This product has been referenced in:
  • Cheng CJ & Huang CL Activation of PI3-Kinase Stimulates Endocytosis of ROMK via Akt1/SGK1-Dependent Phosphorylation of WNK1. J Am Soc Nephrol 22:460-71 (2011). WB ; Human . Read more (PubMed: 21355052) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Hek 293T)
Loading amount 60 µg
Specification Hek 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 18 2010

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