概述

  • 产品名称Anti-WNK1抗体
    参阅全部 WNK1 一抗
  • 描述
    兔多克隆抗体to WNK1
  • 经测试应用适用于: WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from huma WNK1 around the phosphorylation site of threonine 58 (R-H-T-M-D).

  • 阳性对照
    • EGF-treated 293 cell extract; human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Predicted molecular weight: 251 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20398666

靶标

  • 功能Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • 组织特异性Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • 疾病相关Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Erythrocyte 65 kDa protein antibody
    • HSAN2 antibody
    • HSN2 antibody
    • hWNK1 antibody
    • KDP antibody
    • KIAA0344 antibody
    • Kinase deficient protein antibody
    • MGC163339 antibody
    • MGC163341 antibody
    • p65 antibody
    • PRKWNK1 antibody
    • Prostate derived sterile 20 like kinase antibody
    • Protein kinase lysine deficient 1 antibody
    • Protein kinase lysine-deficient 1 antibody
    • Protein kinase with no lysine 1 antibody
    • PSK antibody
    • Serine/threonine protein kinase WNK1 antibody
    • Serine/threonine-protein kinase WNK1 antibody
    • With no K antibody
    • WNK lysine deficient protein kinase 1 antibody
    • WNK1 antibody
    • WNK1_HUMAN antibody
    see all

Anti-WNK1 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with ab53151 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-WNK1 antibody (ab53151) at 1/300 dilution

    Lane 1 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with no peptide
    Lane 2 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with peptide


    Predicted band size : 251 kDa

Anti-WNK1 antibody (ab53151)参考文献

This product has been referenced in:
  • Park HW  et al. Dynamic regulation of CFTR bicarbonate permeability by [Cl-]i and its role in pancreatic bicarbonate secretion. Gastroenterology 139:620-31 (2010). IHC-Fr ; Human . Read more (PubMed: 20398666) »

See 1 Publication for this product

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I'm really sorry but we unfortunately don't. Sorry I cannot help you more.

Thank you for your enquiry. I would predict that a cytoplasmic lysate of human breast tumor tissue will have some WNK1 protein as in tissue sections of human breast carcinoma this protein was detected with the antibody. However, as the lysate is a ho...

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