Withaferin A from Withania somnifera (ab120644)

概述

  • 产品名称Withaferin A from Withania somnifera
  • 描述
    Naturally occurring withanolide/steroidal lactone with anticancer properties
  • 生物学描述Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
  • 纯度> 97%

性能

  • 化学名称(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
  • 分子量470.60
  • 化学结构Chemical Structure
  • 分子式C28H38O6
  • CAS编号5119-48-2
  • 存放说明Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • 溶解度概述Soluble in ethanol
  • 处理

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • 来源

    Withania somnifera

  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab120644 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Withaferin A from Withania somnifera 图像

    • ab7973 staining Bcl2 in HEK 293 cells treated with withaferin A from Withania somnifera (ab120644), by ICC/IF. Decrease in Bcl2 expression with increased concentration of withaferin A from Withania somnifera, as described in literature.
      The cells were incubated at 37°C for 3h in media containing different concentrations of ab120644 (withaferin A from Withania somnifera) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7973 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    Withaferin A from Withania somnifera (ab120644)参考文献

    This product has been referenced in:
    • Zhang X  et al. Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines CaOV3 and SKOV3 by natural withanolide Withaferin A. Gynecol Oncol 124:606-12 (2012). Read more (PubMed: 22188785) »
    • Mirjalili MH  et al. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 14:2373-93 (2009). Read more (PubMed: 19633611) »

    See 0 Publications for this product

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