概述

性能

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应用

Our Abpromise guarantee covers the use of ab38317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25 - 1/100.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA 1/1000.

靶标

  • 功能May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
  • 组织特异性Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
  • 序列相似性Belongs to the CCN family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCN family member 5 antibody
    • CCN5 antibody
    • Connective tissue growth factor like protein antibody
    • Connective tissue growth factor related protein 58 antibody
    • Connective tissue growth factor-like protein antibody
    • Connective tissue growth factor-related protein 58 antibody
    • CT58 antibody
    • CTGF L antibody
    • CTGF-L antibody
    • WISP 2 antibody
    • WISP-2 antibody
    • Wisp2 antibody
    • WISP2_HUMAN antibody
    • Wnt 1 signaling pathway protein 2 antibody
    • WNT1 inducible signaling pathway protein 2 antibody
    • WNT1-inducible-signaling pathway protein 2 antibody
    see all

Anti-WISP2 antibody 图像

  • Anti-WISP2 antibody (ab38317) at 1/1000 dilution + A549 whole cell lysate at 35 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Additional bands at : 50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab38317 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-WISP2 antibody (ab38317) at 1/100 dilution + Recombinant human WISP2 protein (ab50040) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Anti-WISP2 antibody (ab38317)参考文献

This product has been referenced in:
  • Xiao G  et al. The expression of Wnt-1 inducible signaling pathway protein-2 in astrocytoma: Correlation between pathological grade and clinical outcome. Oncol Lett 9:235-240 (2015). Read more (PubMed: 25435966) »
  • Sabbah M  et al. CCN5, a Novel Transcriptional Repressor of the Transforming Growth Factor {beta} Signaling Pathway. Mol Cell Biol 31:1459-69 (2011). IHC-P ; Human . Read more (PubMed: 21262769) »

See all 3 Publications for this product

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