The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25 - 1/100.
Use a concentration of 1 - 5 µg/ml.
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
Belongs to the CCN family. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.
ICC/IF image of ab38317 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-WISP2 antibody (ab38317)
Anti-WISP2 antibody (ab38317) at 1/100 dilution + Recombinant human WISP2 protein (ab50040) at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Xiao G et al. The expression of Wnt-1 inducible signaling pathway protein-2 in astrocytoma: Correlation between pathological grade and clinical outcome. Oncol Lett9:235-240 (2015).
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Chowdhury S et al. IGF-I stimulates CCN5/WISP2 gene expression in pancreatic ß-cells, which promotes cell proliferation and survival against streptozotocin. Endocrinology155:1629-42 (2014).
Read more (PubMed: 24552398) »