Anti-Wilms Tumor Protein抗体(ab51951)

概述

  • 产品名称Anti-Wilms Tumor Protein抗体
    参阅全部 Wilms Tumor Protein 一抗
  • 描述
    山羊多克隆抗体to Wilms Tumor Protein
  • 特异性ab51951 is expected to recognise all four reported isoforms of WT1.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    QDPASTCVPEPASQH

    , corresponding to N terminal amino acids 2-16 of Human Wilms Tumor Protein

  • 阳性对照
    • Human Kidney, Spleen and Testis lysates

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/4000.

靶标

  • 功能Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • 组织特异性Expressed in the kidney and a subset of hematopoietic cells.
  • 疾病相关Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • 序列相似性Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • WIT 2 antibody
    • WT 1 antibody
    • AWT1 antibody
    • FWT1 antibody
    • GUD antibody
    • NPHS4 antibody
    • WAGR antibody
    • Wilms tumor 1 antibody
    • Wilms Tumor antibody
    • Wilms tumor protein antibody
    • Wilms' tumor gene antibody
    • Wilms' tumor protein antibody
    • WIT2 antibody
    • WT antibody
    • WT1 antibody
    • WT1_HUMAN antibody
    • WT33 antibody
    see all

Anti-Wilms Tumor Protein antibody 图像

  • Anti-Wilms Tumor Protein antibody (ab51951) at 1 µg/ml + 35 µg of Human Spleen lysate (in RIPA buffer)

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

Anti-Wilms Tumor Protein antibody (ab51951)参考文献

ab51951 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human pluripotent SC derived epicardial like cells)
Permeabilization Yes - Saponin
Specification Human pluripotent SC derived epicardial like cells
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative Paraformaldehyde
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提交于 Nov 11 2016

ab51951 is a polyclonal antibody and we would therefore expect a mixture of IgG subtypes in this antibody product.


Goats have to my knowledge only two IgG subtypes: IgG1 and IgG2. IgG2 is ca 10-20% of total IgG in goat serum.

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