Anti-Wilms Tumor Protein抗体(ab15249)

概述

  • 产品名称Anti-Wilms Tumor Protein抗体
    参阅全部 Wilms Tumor Protein 一抗
  • 描述
    兔多克隆抗体to Wilms Tumor Protein
  • 经测试应用适用于: IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide (Human) (C terminal).

性能

应用

Our Abpromise guarantee covers the use of ab15249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P 1/100.

靶标

  • 功能Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • 组织特异性Expressed in the kidney and a subset of hematopoietic cells.
  • 疾病相关Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • 序列相似性Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • WIT 2 antibody
    • WT 1 antibody
    • AWT1 antibody
    • FWT1 antibody
    • GUD antibody
    • NPHS4 antibody
    • WAGR antibody
    • Wilms tumor 1 antibody
    • Wilms Tumor antibody
    • Wilms tumor protein antibody
    • Wilms' tumor gene antibody
    • Wilms' tumor protein antibody
    • WIT2 antibody
    • WT antibody
    • WT1 antibody
    • WT1_HUMAN antibody
    • WT33 antibody
    see all

Anti-Wilms Tumor Protein antibody 图像

  • Immunohistochemical analysis of murine kidney tissue, staining Wilms Tumor Protein (red) with ab15249 at 1/300 dilution. A fluorescent-conjugated secondary antibody was used to detect staining.

Anti-Wilms Tumor Protein antibody (ab15249)参考文献

This product has been referenced in:

See all 9 Publications for this product

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The exact immunogen sequence is confidential, but it is contained within the range between aa 389-449 of P19544 (Uniprot), isoform 1.

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For ab15429 we have found that there are problems with this antibody in western blot and that we can no longer guarantee that it will work in this application. However it is still guaranteed to work in IP and IHC-P....

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Thank you for sending a copy of the original order for ab15249. The order only shows that 1 vial of ab15249 was ordered, could you send me the order for the second vial please. I can process the request for 1 replacement vial now so that you can get it...

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