兔多克隆抗体to WIF1 - N-terminal
Synthetic peptide from the N-terminal region of human WIF1, conjugated to KLH.
293 cell lysates transiently transfected with the WIF1 gene.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
The antibody was purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 42 kDa.
1/10 - 1/50.
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Contains 5 EGF-like domains.
Contains 1 WIF domain.
Information by UniProt
Wnt inhibitory factor 1 antibody
Western blot - Anti-WIF1 antibody - N-terminal (ab71204)
All lanes : Anti-WIF1 antibody - N-terminal (ab71204) at 1/50 dilution Lane 1 : Nontransfected 293 cell lysates Lane 2 : 293 cell lysates transiently transfected with the WIF1 gene Lysates/proteins at 2 µg per lane. Predicted band size: 42 kDa Observed band size: 42 kDa Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIF1 antibody - N-terminal (ab71204)
ab71204 at 1/50 dilution staining WIF1- N-terminal in human Lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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