概述

  • 产品名称
  • 描述
    兔多克隆抗体to WIF1
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human WIF1.

    (Peptide available as ab33600.)

  • 阳性对照
    • ab33281 gave a positive signal in the Human WIF-1 recombinant.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human WIF1 peptide (ab33600).

靶标

Anti-WIF1 antibody 图像

  • Anti-WIF1 antibody (ab33281) at 1/250 dilution + WIF-1 Recombinant Protein at 0.0025 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    The band we observe at 46 kDa is believed to be the glycosylated form of the recombinant protein.

Anti-WIF1 antibody (ab33281)参考文献

This product has been referenced in:
  • De Pauw A  et al. Dnmt3a-mediated inhibition of Wnt in cardiac progenitor cells improves differentiation and remote remodeling after infarction. JCI Insight 2:N/A (2017). WB ; Mouse . Read more (PubMed: 28614798) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hair follicle)
Permeabilization
No
Specification
Hair follicle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Ahmar Aziz

Verified customer

提交于 May 19 2015

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (hek293, overexpression of hWIF1)
Specification
hek293, overexpression of hWIF1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 May 20 2013

Thank you very much for your interest in ab33281. To our knowledge, ab33281 has not been tested in immunofluorescence. Therefore, I can offer a discount off a future purchase if you buy ab33281 now, test it in immunofluorescence and submit feedback to ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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