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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human WIF1.
(Peptide available as ab33600.)
Our Abpromise guarantee covers the use of ab33281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/250. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human WIF1 peptide (ab33600).|
ab33281 staining WIF1 in Mouse MEF cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 5% Goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in 0.5% Triton X-100) for 2 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
ab33281 has not yet been referenced specifically in any publications.