The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user.
Proteolytic enzymatic pretreatment of the tissue sections is recommended prior to immunostaining.