概述

  • 产品名称
  • 描述
    兔多克隆抗体to WIBG
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 15-44 of Human WIBG (NP_115721.1), conjugated to KLH.

  • 阳性对照
    • Mouse spleen tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab108152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 23 kDa.

靶标

  • 功能
    Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.
  • 序列相似性
    Belongs to the pym family.
  • 结构域
    The eIF2A-like region shares sequence similarity with eIF2A and mediates the interaction with the 40S ribosomal subunit and the 48S preinitiation complex.
  • 细胞定位
    Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Shuttles between the nucleus and the cytoplasm (PubMed:14968132). Nuclear export is mediated by XPO1/CRM1 (PubMed:14968132).
  • Information by UniProt
  • 数据库链接
  • 别名
    • A030010B05Rik antibody
    • MGC13064 antibody
    • Partner of Y14 and mago antibody
    • Protein wibg homolog antibody
    • PYM antibody
    • PYM homolog 1 exon junction complex-associated factor antibody
    • pym1 antibody
    • PYM1_HUMAN antibody
    • Within bgcn homolog antibody
    see all

Anti-WIBG antibody 图像

  • Anti-WIBG antibody (ab108152) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 23 kDa

实验方案

Anti-WIBG antibody (ab108152)参考文献

This product has been referenced in:
  • Chuang TW  et al. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem 291:8565-74 (2016). Read more (PubMed: 26887951) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. We will guarantee this antibody to react with any tissue or cell lysates where human or mouse WIBG is expressed. If you have any trouble using this antibody on HeLa or 293T cell lysates, please contact us within 6 months an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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