WI-38全细胞裂解液(ab3960)

概述

  • 产品名称WI-38全细胞裂解液
  • 常规说明Cell line: WI-38 (Human lung fibroblast, normal).
    Growth media: Minimum essential medium (Eagle) with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, and 10% FBS.


    WI-38 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 裂解物说明WI-38 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab3960 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. Ready to load; 10 - 20 µg/lane is recommended for mini gel.

    WI-38 whole cell lysate (ab3960)参考文献

    This product has been referenced in:
    • Weitzel LR  et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat 124:297-305 (2010). Read more (PubMed: 20087651) »

    See 1 Publication for this product

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    Thank you for contacting Abcam and for your interest in our whole cell lysates.

    Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"