To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
The protein content has been determined by a Bradford-based assay.
The WI-38 nuclear extracts have been collected after a 2-hour incubation with or without TPA (100 ng/ml) and ionomycin (calcium ionophore) (2 µg/ml).
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.