WI-38核extract裂解液(forskolin stimulated) (ab14912)

概述

  • 产品名称WI-38核extract裂解液(forskolin stimulated)
    参阅全部 WI 38 细胞裂解液
  • 描述
    WI-38核extract裂解液
  • 常规说明The WI-38 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay.

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitor
  • Concentration information loading...
  • 研究领域

    WI-38 nuclear extract lysate (forskolin stimulated) 图像

    • Each lot has been tested for CREB activation by using TransAM™ pCREB Kits. The signal intensity for CREB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 3. This ratio may vary depending on the basal level of CREB activation in a given cell type.

    WI-38 nuclear extract lysate (forskolin stimulated) (ab14912)参考文献

    ab14912 has not yet been referenced specifically in any publications.

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