概述

  • 产品名称Anti-WHSC2抗体
    参阅全部 WHSC2 一抗
  • 描述
    小鼠多克隆抗体to WHSC2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human WHSC2 protein (NP_005654.2).

  • 阳性对照
    • WB: Jurkat cell lysate; WHSC2 transfected 293T cell lysate ICC/IF: HeLa cell

性能

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应用

Our Abpromise guarantee covers the use of ab88870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
  • 组织特异性Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
  • 序列相似性Belongs to the NELF-A family.
    Contains 1 HDAg-like domain.
  • 结构域The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ10442 antibody
    • FLJ25112 antibody
    • Negative elongation factor A antibody
    • Negative elongation factor polypeptide A antibody
    • NELF A antibody
    • NELF-A antibody
    • NELFA antibody
    • NELFA_HUMAN antibody
    • P/OKcl.15 antibody
    • P/OKcl15 antibody
    • WHSC 2 antibody
    • Whsc2 antibody
    • Wolf Hirschhorn syndrome candidate 2 antibody
    • Wolf Hirschhorn syndrome candidate 2 protein antibody
    • Wolf-Hirschhorn syndrome candidate 2 protein antibody
    see all

Anti-WHSC2 antibody 图像

  • Anti-WHSC2 antibody (ab88870) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • All lanes : Anti-WHSC2 antibody (ab88870) at 1 µg/ml

    Lane 1 : WHSC2 transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 58 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab88870, at 10 µg/ml, staining WHSC2 in HeLa cell by Immunofluorescence.

Anti-WHSC2 antibody (ab88870)参考文献

ab88870 has not yet been referenced specifically in any publications.

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