概述

  • 产品名称Anti-WHSC1/NSD2抗体
    参阅全部 WHSC1/NSD2 一抗
  • 描述
    兔多克隆抗体to WHSC1/NSD2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Dog
  • 免疫原

    Synthetic peptide, corresponding to amino acids 219-268 of Human WHSC1

  • 阳性对照
    • HepG2 cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab28470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

靶标

  • 功能Probable histone methyltransferase (By similarity). May act as a transcription regulator that binds DNA and suppresses IL5 transcription.
  • 组织特异性Widely expressed.
  • 疾病相关Note=A chromosomal aberration involving WHSC1 is a cause of multiple myeloma tumors. Translocation t(4;14)(p16.3;q32.3) with IgH.
    Note=WHSC1 is located in the Wolf-Hirschhorn syndrome (WHS) critical region. WHS results from by sub-telomeric deletions in the short arm of chromosome 4. WHSC1 is deleted in every case, however deletion of linked genes contributes to both the severity of the core characteristics and the presence of the additional syndromic problems.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 HMG box DNA-binding domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm and Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ23286 antibody
    • IL5 promoter REII region binding protein antibody
    • KIAA1090 antibody
    • MGC176638 antibody
    • MMSET antibody
    • MMSET type II antibody
    • Multiple myeloma SET domain containing protein type III antibody
    • Multiple myeloma SET domain protein antibody
    • Multiple myeloma SET domain-containing protein antibody
    • NSD 2 antibody
    • NSD2 antibody
    • NSD2_HUMAN antibody
    • Nuclear SET domain-containing protein 2 antibody
    • Probable histone-lysine N-methyltransferase NSD2 antibody
    • Protein trithorax-5 antibody
    • REIIBP antibody
    • Trithorax/ash1 related protein 5 antibody
    • TRX5 antibody
    • TRX5 protein antibody
    • WHS antibody
    • Whsc1 antibody
    • Wolf Hirschhorn syndrome candidate 1 antibody
    • Wolf Hirschhorn syndrome candidate 1 protein antibody
    • Wolf-Hirschhorn syndrome candidate 1 protein antibody
    see all

Anti-WHSC1/NSD2 antibody 图像

  • Anti-WHSC1/NSD2 antibody (ab28470) at 0.5 µg/ml + 5% skim milk / PBS buffer.
    Developed using the ECL technique

    Predicted band size : 72 kDa

Anti-WHSC1/NSD2 antibody (ab28470)参考文献

This product has been referenced in:
  • Hajdu I  et al. Wolf-Hirschhorn syndrome candidate 1 is involved in the cellular response to DNA damage. Proc Natl Acad Sci U S A 108:13130-4 (2011). WB ; Human . Read more (PubMed: 21788515) »

See 1 Publication for this product

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To our knowledge, ab28470 has not been tested in mouse. We predict it to work in mouse due to high sequence homology, but we do not guarantee it since we haven't tested this. Therefore, I can o...

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Application Western blot
Sample Human Cell lysate - whole cell (Hela & HCT116 cells)
Loading amount 50 µg
Specification Hela & HCT116 cells
Gel Running Conditions Reduced Denaturing (10% tris glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 May 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"