概述

  • 产品名称
  • 描述
    兔多克隆抗体to WHIP
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    SRESYDAPPTPSGAC

    conjugated to KLH, corresponding to amino acids 107-120 of Human WHIP.

性能

应用

Our Abpromise guarantee covers the use of ab4731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
  • 应用说明
    ELISA: 1/5000 - 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 17888034).
    WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
    • 组织特异性
      Ubiquitously expressed.
    • 序列相似性
      Belongs to the AAA ATPase family.
      Contains 1 UBZ-type zinc finger.
    • 翻译后修饰
      Sumoylated by SUMO1 and SUMO2/3.
    • 细胞定位
      Nucleus. Colocalizes with WRN in granular structures in the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATPase WRNIP 1 antibody
      • ATPase WRNIP1 antibody
      • bA420G6.2 antibody
      • FLJ22526 antibody
      • Putative helicase RUVBL antibody
      • RP11 420G6.2 antibody
      • Werner helicasae interacting protein 1 antibody
      • Werner helicasae interacting protein antibody
      • Werner helicase interacting protein isoform 2 antibody
      • Werner helicase-interacting protein 1 antibody
      • WRIP1_HUMAN antibody
      • WRNIP 1 antibody
      • WRNIP antibody
      • Wrnip1 antibody
      see all

    Anti-WHIP antibody 图像



    • Predicted band size : 72 kDa


      Western blot using ab4731 on HEK 293 lysate at 1/200.

      ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. The main band could be blocked using the immunising peptide.

    Anti-WHIP antibody (ab4731)参考文献

    This product has been referenced in:
    • Mano Y  et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . Read more (PubMed: 17888034) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293)
    Loading amount
    10 µg
    Specification
    293
    Treatment
    lentivirus transduced for RNAi
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    提交于 Mar 05 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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