The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. This polyclonal antibody can be used for the detection of the WNV Matrix precursor protein in ELISA. It will detect 10 ng of free peptide at 1 µg/ml.
Use at an assay dependent dilution. PubMed: 17634508
Use at an assay dependent concentration. PubMed: 21697345
West Nile Virus is part of the flavivirus family, many of which are capable of causing serious illness in humans. The ssRNA + strand virus is enveloped and spherical with a diameter of 40-50 nm, similar to all flaviviruses.
The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E. Inside cells, viral particles will contain a preM particle and E in a complex that matures through proteolytic cleavage and processing in the Golgi apparatus.
WNV has three different effects on humans, the first is asymptomatic infection; the second is a mild febrile syndrome termed West Nile Fever; the third is a neuroinvasive disease termed West Nile meningitis or encephalitis. In infected individuals the ratio between the three states is roughly 110:30:1. The main route of human infection is through the bite of an infected mosquito.