Anti-West Nile Virus M glycoprotein抗体(ab22070)

概述

  • 产品名称Anti-West Nile Virus M glycoprotein抗体
    参阅全部 West Nile Virus M glycoprotein 一抗
  • 描述
    兔多克隆抗体to West Nile Virus M glycoprotein
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    Reacts with West Nile Virus. Not yet tested in other species.
  • 免疫原

    Synthetic peptide corresponding to West Nile Virus M glycoprotein aa 8-27.
    Sequence:

    GESTLANKKGAWLDSTKATR

  • 阳性对照
    • West Nile virus infected cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab22070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 8 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 17634508

靶标

  • 相关性West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.
  • 别名
    • Envelope protein M antibody
    • Matrix protein antibody
    • West Nile virus matrix protein antibody
    • WNV matrix protein antibody

Anti-West Nile Virus M glycoprotein antibody 图像

  • IHC analysis of WNV-Envelope protein in bird liver using ab22070 at 1ug/ ml incubated for 30 minutes. A secondary conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. Image courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine

  • Western blot analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected cells using ab22070 at 1 ug/ml.

Anti-West Nile Virus M glycoprotein antibody (ab22070)参考文献

This product has been referenced in:
  • Mukherjee S  et al. Mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol 88:7210-20 (2014). Read more (PubMed: 24741083) »
  • Velado Fernández I  et al. Development of a novel protocol for generating flavivirus reporter particles. J Virol Methods 208:96-101 (2014). Read more (PubMed: 25116200) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Cell lysate - whole cell (killed virus)
Loading amount 0.03 µg
Specification killed virus
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
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提交于 Jul 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"