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Our Abpromise guarantee covers the use of ab22070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 8 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17634508|
IHC analysis of WNV-Envelope protein in bird liver using ab22070 at 1ug/ ml incubated for 30 minutes. A secondary conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. Image courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine