使用敲除细胞株进行验证RabMAb

Anti-Werner's syndrome helicase WRN抗体[EPR6392] (ab124673)

概述

  • 产品名称Anti-Werner's syndrome helicase WRN抗体[EPR6392]
    参阅全部 Werner's syndrome helicase WRN 一抗
  • 描述
    兔单克隆抗体[EPR6392] to Werner's syndrome helicase WRN
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to C-terminal amino acids of Human Werner's syndrome helicase WRN.

  • 阳性对照
    • MOLT 4, K562 and A431 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
    • 疾病相关Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
      Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
    • 序列相似性Belongs to the helicase family. RecQ subfamily.
      Contains 1 3'-5' exonuclease domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.
    • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus > nucleolus. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686C2056 antibody
      • DNA helicase antibody
      • DNA helicase, RecQ like type 3 antibody
      • Exonuclease WRN antibody
      • HGNC 12791 antibody
      • OTTHUMP00000225301 antibody
      • RecQ protein-like 2 antibody
      • RecQ-like type 3 antibody
      • RecQ3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • Werner syndrome ATP-dependent helicase antibody
      • Werner syndrome helicase antibody
      • Werner syndrome protein antibody
      • Werner syndrome, RecQ helicase like antibody
      • WRN antibody
      • WRN_HUMAN antibody
      see all

    Anti-Werner's syndrome helicase WRN antibody [EPR6392] 图像



    • Predicted band size : 162 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 µg)
      Lane 3: MOLT4 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 to 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
      ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 162 kDa

    Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)参考文献

    ab124673 has not yet been referenced specifically in any publications.

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