Anti-Werner's syndrome helicase WRN抗体(ab17988)

概述

  • 产品名称Anti-Werner's syndrome helicase WRN抗体
    参阅全部 Werner's syndrome helicase WRN 一抗
  • 描述
    兔多克隆抗体to Werner's syndrome helicase WRN
  • 经测试应用适用于: WB, IPmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    The epitope recognised to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191.

  • 阳性对照
    • western blot on 293Tcells, whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab17988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at a concentration of 2 - 5 µg/ml.
    WB: 1/5,000 - 1/25,000. Detects a band of approximately 200 kDa (predicted molecular weight: 159 kDa).

    ab17988 does not seem to be suitable for ICC/IF - please refer to abreview for further details.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
    • 疾病相关Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
      Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
    • 序列相似性Belongs to the helicase family. RecQ subfamily.
      Contains 1 3'-5' exonuclease domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.
    • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus > nucleolus. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686C2056 antibody
      • DNA helicase antibody
      • DNA helicase, RecQ like type 3 antibody
      • Exonuclease WRN antibody
      • HGNC 12791 antibody
      • OTTHUMP00000225301 antibody
      • RecQ protein-like 2 antibody
      • RecQ-like type 3 antibody
      • RecQ3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • Werner syndrome ATP-dependent helicase antibody
      • Werner syndrome helicase antibody
      • Werner syndrome protein antibody
      • Werner syndrome, RecQ helicase like antibody
      • WRN antibody
      • WRN_HUMAN antibody
      see all

    Anti-Werner's syndrome helicase WRN antibody 图像

    • All lanes : Anti-Werner's syndrome helicase WRN antibody (ab17988) at 1/1000 dilution

      Lane 1 : Human Lymphocyte whole cell lysate
      Lane 2 : Human Lymphocyte whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      Anti Rabbit IgG conjugated to HRP at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 159 kDa


      Exposure time : 10 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    • Detection of Human WRN by western blot and immunoprecipitation.

      Lane 1: 100 µg of whole cell lysate from 293T cells.
      Lane 2: 293T cell lysate immunoprecipated using IgG.
      Lane 3: 293T cell lysate immunoprecipitated using 5 µg of ab17987 + WRN blocking peptide.
      Lane 4: 293T cell lysate immunoprecipitated using 5 µg of ab17987.
      Lane 5: 293T cell lysate immunoprecipitated using 5 µg of ab17988 +WRN blocking peptide.
      Lane 6:  293T cell lysate immunoprecipitated using 5 µg of ab17988.

      Lanes 2-6 One 10 cm plate of 293T cells used for immunoprecipitation. 

      Anti-WRN antibody (ab17988) used at 1/10,000 for western blot.
      Detection:  Chemiluminescence with an exposure time of ~10 seconds.

    Anti-Werner's syndrome helicase WRN antibody (ab17988)参考文献

    This product has been referenced in:
    • Sallmyr A  et al. Up-regulation of WRN and DNA ligase III{alpha} in chronic myeloid leukemia: consequences for the repair of DNA double strand breaks. Blood : (2008). Read more (PubMed: 18524993) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (lymphocytes)
    Specification lymphocytes
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 05 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (RPE-1)
    Specification RPE-1
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Aug 16 2007

    Thank you for getting back in touch with me with your order details. I have arranged for a credit note to be raised by our accounts department against order numbers 126743 and 114423 for two vials of ab17988. They will be in touch with your purchas...

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    Thank you for your enquiry. In Charlotte's absence I am dealing with her enquiries. I am sorry to hear that you have been having difficulties with these antibodies. I have been reading through your technical questionaires and I appreciate the de...

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    Thank you for your enquiry. You ask an interesting question. I have been in discussion with the lab and they have referred me to the fact that the resolution of proteins by SDS-PAGE does not define molecular weight. It defines the relative mobilit...

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