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The epitope recognised to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Detection of Human WRN by western blot and immunoprecipitation.
Lane 1: 100
Lane 2: 293T cell lysate immunoprecipated using IgG.
Lane 3: 293T cell lysate immunoprecipitated using 5
Lane 4: 293T cell lysate immunoprecipitated using 5
Lane 5: 293T cell lysate immunoprecipitated using 5
Lane 6: 293T cell lysate immunoprecipitated using 5
Lanes 2-6 One 10 cm plate of 293T cells used for immunoprecipitation.
Anti-WRN antibody (ab17988) used at 1/10,000 for western blot.
Detection: Chemiluminescence with an exposure time of ~10 seconds.
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