Anti-Werner's syndrome helicase WRN抗体(ab17987)

概述

  • 产品名称Anti-Werner's syndrome helicase WRN抗体
    参阅全部 Werner's syndrome helicase WRN 一抗
  • 描述
    兔多克隆抗体to Werner's syndrome helicase WRN
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The epitope recognized by ab17987 maps to a region between residues 400 and 450 of human Werner Syndrome Helicase.

  • 阳性对照
    • Whole cell lysate from 293T cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab17987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • 疾病相关Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 序列相似性Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleolus. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686C2056 antibody
    • DNA helicase antibody
    • DNA helicase, RecQ like type 3 antibody
    • Exonuclease WRN antibody
    • HGNC 12791 antibody
    • OTTHUMP00000225301 antibody
    • RecQ protein-like 2 antibody
    • RecQ-like type 3 antibody
    • RecQ3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • Werner syndrome ATP-dependent helicase antibody
    • Werner syndrome helicase antibody
    • Werner syndrome protein antibody
    • Werner syndrome, RecQ helicase like antibody
    • WRN antibody
    • WRN_HUMAN antibody
    see all

Anti-Werner's syndrome helicase WRN antibody 图像

  • ab17987, at 0.1mcg/ml, staining Human WRN in whole cell lysate (100mcg) in 293T cells by Western blot. WRN was immunoprecipitated with ab17987 at 5mcg/10cm plate (+/- blocking peptide). Detection was by chemiluminescence with an exposure time of less than 10 seconds.

实验方案

Anti-Werner's syndrome helicase WRN antibody (ab17987)参考文献

This product has been referenced in:
  • Otterlei M  et al. Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest. J Cell Sci 119:5137-46 (2006). WB, IP ; Human . Read more (PubMed: 17118963) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (lymphocytes)
Specification lymphocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 05 2014

Thank you for your enquiry. As you know, the molecular weight of BSA is expected to be 67kDa, which is lower that the 75-105kDa band area that you observed. In addition, I have confirmed with the originator of this product that it does not contain B...

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Thank you for your enquiry. I can confirm that this product is supposed to detect an approximately 200 kDa band and there are no BSA included in the product. In your previous submitted Abreview, you mentioned that you were able to obtain a specific...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells and WSKO372 lymphoblastoid cells)
Loading amount 10 µg
Specification Jurkat cells and WSKO372 lymphoblastoid cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Miss. mari sild

Verified customer

提交于 Sep 27 2006

Thank you for your enquiry. You ask an interesting question. I have been in discussion with the lab and they have referred me to the fact that the resolution of proteins by SDS-PAGE does not define molecular weight. It defines the relative mobilit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"