Anti-WEE2抗体(ab121943)

概述

  • 产品名称Anti-WEE2抗体
    参阅全部 WEE2 一抗
  • 描述
    兔多克隆抗体to WEE2
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: TKSGNHFEEP KLKDILLQIS LGLNYIHNSS MVHLDIKPSN IFICHKMQSE SSGVIEEVEN EADWFLSANV MYKIGDLGHA TSINKPKVEE GD, corresponding to amino acids 305-396 of Human WEE2.

  • 阳性对照
    • A431 cells; Human duodenum tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/700. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
  • 组织特异性Expressed in testis.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation leads to increase its activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hCG_39453 antibody
    • WEE1 homolog 2 (S. pombe) antibody
    • Wee1-like protein kinase 1B antibody
    • Wee1-like protein kinase 2 antibody
    • WEE1B antibody
    • Wee1B kinase antibody
    • Wee2 antibody
    • WEE2_HUMAN antibody
    see all

Anti-WEE2 antibody 图像

  • ab121943, at 1/700 dilution, staining WEE2 in paraffin embedded Human duodenum tissue by Immunohistochemistry.
  • ab121943, at 4 µg/ml, staining WEE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Anti-WEE2 antibody (ab121943)参考文献

ab121943 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (ovary)
Permeabilization Yes - 0.3% Triton X-100, 20 min
Specification ovary
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
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提交于 Mar 23 2015

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The immunogen of ab121943 shows only 82% homology with mouse. We generally expect a cross reactivity from 85% homology. This is a border line case and I cannot recommend using this antibody in this case since WB i...

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