Recombinant fragment, corresponding to a region within amino acids 212-418 of Human WDR85 (AAH17335).
IMR32 cell lysate. Gastric carcinoma. tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 1% BSA, 20% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.
Plays a role in the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.
Belongs to the WD repeat WDR85 family.
Contains 3 WD repeats.
Information by UniProt
Diphthamide biosynthesis 7 antibody
Western blot - Anti-WDR85 antibody (ab111910)
Anti-WDR85 antibody (ab111910) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 51 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR85 antibody (ab111910)
ab111910 at 1/500 dilution staining WDR85 in paraffin-embedded gastric carcinoma by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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