概述

  • 产品名称Anti-WDR79抗体
    参阅全部 WDR79 一抗
  • 描述
    兔多克隆抗体to WDR79
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 1 and 50 of Human WDR79 (NP_060551.1)

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab99376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage.
  • 组织特异性Expressed in all tissues and cell lines examined.
  • 疾病相关Defects in WRAP53 are the cause of dyskeratosis congenita autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
  • 序列相似性Contains 6 WD repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > Cajal body. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ10385 antibody
    • TCAB1 antibody
    • Telomerase Cajal body protein 1 antibody
    • WAP53_HUMAN antibody
    • WD repeat containing protein 79 antibody
    • WD repeat-containing protein 79 antibody
    • WD40 repeat-containing protein encoding RNA antisense to p53 antibody
    • Wrap53 antibody
    see all

Anti-WDR79 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling WDR79 with ab99376 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-WDR79 antibody (ab99376) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 59 kDa


    Exposure time : 30 seconds
  • Lane 1 : Detection of Human WDR79 by Immunoprecipitation from HeLa cells(1 mg for IP, 20% of IP loaded), using 3µg/mg lysate . Lane 2 : Control IgG

Anti-WDR79 antibody (ab99376)参考文献

This product has been referenced in:
  • Yuan P  et al. Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to telomeres and induces G1 cell cycle arrest in A549 cells. Oncol Lett 8:1009-1016 (2014). WB ; Human . Read more (PubMed: 25120649) »
  • Jády BE  et al. Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs. Genes Dev 26:1897-910 (2012). Read more (PubMed: 22892240) »

See all 2 Publications for this product

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