概述

  • 产品名称Anti-WDR79抗体
    参阅全部 WDR79 一抗
  • 描述
    小鼠单克隆抗体to WDR79
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Cynomolgus Monkey, Macaque Monkey
  • 免疫原

    Recombinant fragment: AVSQELREGD PVSLSTPLET EFGSPSELSP RIEEQELSEN TSLPAEEANG SLSEEEANGP ELGSGKAMED TSGEPAAEDE GDTAWNYSFS QLPRFLSGS, corresponding to amino acids 62-160 of Human WDR79 (NP_060551), with a proprietary tag.

  • 阳性对照
    • A431 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage.
  • 组织特异性Expressed in all tissues and cell lines examined.
  • 疾病相关Defects in WRAP53 are the cause of dyskeratosis congenita autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
  • 序列相似性Contains 6 WD repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > Cajal body. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ10385 antibody
    • TCAB1 antibody
    • Telomerase Cajal body protein 1 antibody
    • WAP53_HUMAN antibody
    • WD repeat containing protein 79 antibody
    • WD repeat-containing protein 79 antibody
    • WD40 repeat-containing protein encoding RNA antisense to p53 antibody
    • Wrap53 antibody
    see all

Anti-WDR79 antibody 图像

  • Anti-WDR79 antibody (ab77333) at 5 µg/ml + A431 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 59 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
  • Anti-WDR79 antibody (ab77333) at 5 µg/ml + immunogen (tagged 99 aa recombinant fragment) at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 59 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-WDR79 antibody (ab77333)参考文献

ab77333 has not yet been referenced specifically in any publications.

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