Anti-WDR70抗体(ab86366)

概述

  • 产品名称Anti-WDR70抗体
  • 描述
    兔多克隆抗体to WDR70
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 604 and 654 of Human WDR70 (NP_060504.1)

  • 阳性对照
    • HeLa or 293T whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab86366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 序列相似性Belongs to the WD repeat GAD-1 family.
    Contains 7 WD repeats.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4833422F06Rik antibody
    • FLJ10233 antibody
    • MGC109151 antibody
    • RGD1309487 antibody
    • WD repeat containing protein 70 antibody
    • WD repeat domain 70 antibody
    • WD repeat-containing protein 70 antibody
    • wdr70 antibody
    • WDR70_HUMAN antibody
    see all

Anti-WDR70 antibody 图像

  • All lanes : Anti-WDR70 antibody (ab86366) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 73 kDa


    Exposure time : 30 seconds
  • Detection of WDR70 by Western Blot of Immunprecipitate.
    ab86366 at 1µg/ml staining WDR70 in HeLa whole cell lysate immunoprecipitated using ab86366 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-WDR70 antibody (ab86366)参考文献

ab86366 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - 0.5%TritonX
Specification U2OS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative Paraformaldehyde
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提交于 Sep 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"