antigen sequence: PLLSCNLKDD FEFFFHHRNN LDINVVIRQV YHLMETTPTD IHPDSMLNVF VALTKGQYPV FNQYPKFIVD YQTQERERIR NDELDYLRER QTVEDMQAKV, corresponding to amino acids 617-716 of Human WDR67.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Use at an assay dependent concentration. Predicted molecular weight: 124 kDa.
Contains 1 Rab-GAP TBC domain.
Contains 5 WD repeats.
Information by UniProt
WD repeat containing protein 67 antibody
WD repeat domain 67 antibody
Western blot - Anti-WDR67 antibody (ab121771)
All lanes : Lane 1 : Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : Negative control (vector only transfected HEK293T lysate) Lane 3 : Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407919) Predicted band size: 124 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WDR67 antibody (ab121771)
Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab121771 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR67 antibody (ab121771)
ab121771 at 1/50 dilution staining WDR67 in paraffin embedded Human testis tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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