概述

  • 产品名称
    Anti-WDR67抗体
  • 描述
    兔多克隆抗体to WDR67
  • 经测试应用
    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: PLLSCNLKDD FEFFFHHRNN LDINVVIRQV YHLMETTPTD IHPDSMLNVF VALTKGQYPV FNQYPKFIVD YQTQERERIR NDELDYLRER QTVEDMQAKV, corresponding to amino acids 617-716 of Human WDR67.

  • 阳性对照
    • Human testis tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB Use at an assay dependent concentration. Predicted molecular weight: 124 kDa.

靶标

图片

  • Lane 1 :
    Lane 2 :
    Lane 3 :

    Lane 1 : Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : Negative control (vector only transfected HEK293T lysate)
    Lane 3 : Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407919)


    Predicted band size : 124 kDa
  • Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab121771 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121771 at 1/50 dilution staining WDR67 in paraffin embedded Human testis tissue by Immunohistochemistry.

文献

ab121771 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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