概述

  • 产品名称Anti-WDR66抗体
    参阅全部 WDR66 一抗
  • 描述
    兔多克隆抗体to WDR66
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide within residues: GELEEKTDRM PQDELGQERR DLEPENREEG QERRVSDIQS KAGISRESLV , corresponding to N terminal amino acids 179-228 of Human WDR66 (NP_653269).

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 168 kDa (predicted molecular weight: 131 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

Anti-WDR66 antibody 图像

  • Anti-WDR66 antibody (ab83694) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 131 kDa
    Observed band size : 168 kDa (why is the actual band size different from the predicted?)

Anti-WDR66 antibody (ab83694)参考文献

ab83694 has not yet been referenced specifically in any publications.

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