概述

  • 产品名称Anti-WDR48抗体
  • 描述
    兔多克隆抗体to WDR48
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    antigen sequence: SIIQCHILND KRHILTKDTN NNVAYWDVLK ACKVEDLGKV DFEDEIKKRF KMVYVPNWFS VDLKTGMLTI TLDESDCFAA WVSAKDAGF, corresponding to internal sequence amino acids 362-450 of Human WDR48.

  • 阳性对照
    • Human colon tissue; RT 4, U51 MG, Human liver and Human tonsil lysates; A431 cells.

性能

应用

Our Abpromise guarantee covers the use of ab122473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

  • 功能Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the WD repeat WDR48 family.
    Contains 8 WD repeats.
  • 结构域N-terminal WD region interacts with TIP and C-terminal region mediates lysosomal localization. The WD repeats are required for the interaction with deubiquitinating enzymes USP1, USP12 and USP46.
  • 细胞定位Nucleus. Cytoplasm. Lysosome. Mainly cytoplasmic. In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11.
  • Information by UniProt
  • 数据库链接
  • 别名
    • p80 antibody
    • UAF 1 antibody
    • UAF1 antibody
    • USP1 associated factor 1 antibody
    • USP1-associated factor 1 antibody
    • WD repeat containing protein 48 antibody
    • WD repeat domain 48 antibody
    • WD repeat endosomal protein antibody
    • WD repeat-containing protein 48 antibody
    • WDR 48 antibody
    • WDR48 antibody
    • WDR48_HUMAN antibody
    see all

Anti-WDR48 antibody 图像

  • All lanes : Anti-WDR48 antibody (ab122473) at 1/100 dilution

    Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
    Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab122473, at 1/20 dilution, staining WDR48 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-WDR48 antibody (ab122473) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique
  • ab122473, at 4 µg/ml, staining WDR48 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Anti-WDR48 antibody (ab122473)参考文献

ab122473 has not yet been referenced specifically in any publications.

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